Ngā Pūrongo - He aha te Hiranga o te Whakamātautau HPLC mō te Whakapūmau Kounga o te Paura Luteolin?

He aha tonu te whakamātautau HPLC?

Ko te Chromatography Wai Mahi-Teitei (HPLC) he tikanga taiwhanga tika i hangaia hei wehewehe, hei tautuhi, hei ine hoki i ngā matū matū i roto i te ranunga. Mō te paura luteolin—he maha ngā wā ka tangohia mai iSophora japonica(te rākau pagoda Hapanihi) ngā putiputi, ngā kiri pīnati rānei—e kiia ana ko te HPLC te paerewa koura mō te whakatau i te parakore. I roto i te mahi, ka whakarewahia te tauira ki roto i tētahi whakarewa tika, ka panaia mā roto i tētahi pou kua whakakiia ki ngā rauemi tākai motuhake i raro i te pēhanga teitei. Ka rere ngā matū rerekē i ngā tere rerekē, ka taea ai te wehe me te ine i te ngota luteolin. Mā tēnei ka whakarite ko te ōrau parakore kua whakahuatia i runga i te Tiwhikete Tātari (COA) e ahu mai ana i ngā raraunga tika, ka taea te whakaputa anō.

He aha te mea nui rawa atu te parakore mō te paura Luteolin?

Mō ngā kaihoko rahinga—ina koa ko ngā waitohu tāpiringa kai, ngā kamupene rongoā, me ngā kaihanga whakapaipai—ehara te parakore i te mea he tau noa iho; engari e whakatau ana i te whanonga o te kai i roto i te hua kua oti.

Tae me te Āhua: Ko te luteolin tino parakore (≥98%) he kōwhai kanapa te nuinga, ko ngā taumata iti iho te parakore ka ahua mangu, parauri rānei nā ngā tae tipu me ngā rauemi toenga.
Te reka me te kakara: Ka puta te kawa, te kawa rānei i ngā poke.
Te Wairewatanga me te Pūmautanga: Ka pā pea ngā toenga flavonoids, ngā otaota rānei ki te pai o te rewa o te luteolin i roto i ngā whakarewa me te roa o tōna pumau i runga i te whata.
Te Tika o te Inenga: Ko ngā whakatakotoranga i ahu mai i ngā raraunga parakore hē ka hua ake he iti rawa te inenga, he nui rawa rānei te inenga, ka arahi pea ki ngā raruraru ture me ngā whakahokinga mai o ngā hua.

Mā te manatoko i te parakore ki te HPLC, ka taea e ngā kaiwhakarato te kī taurangi ka tutuki i ia kirokaramu o te paura luteolin te kukū tuturu e tumanakohia ana e ngā kaihoko.

He aha te whakataurite o te HPLC ki ētahi atu tikanga whakamātautau?

Ahakoa he tere ake, he iti ake hoki te utu o ngā tikanga pēnei i te ine ira UV, ka inehia e rātou te mimiti i ngā roangaru motuhake—ko te tikanga ka taea e ētahi atu flavonoid he rite te mimiti UV te whakanui ake i te pānui luteolin. Ka whakatauhia e te HPLC tēnei raruraru mā te wehewehe i ia pūhui i mua i te kitenga. Ko te tikanga ko te hua e whakaatu pono ana i te ihirangi luteolin, ehara i te ranunga o ngā ngota rite.

Mō ngā kaihoko me ngā kawemai o te ao, he nui ake te mana o ngā raraunga HPLC, inā koa ka whakaurua ki te taha o ngā chromatograms i roto i te COA. He maha ngā wā ka hiahiatia e ngā rōpū whakahaere, ngā kaitātari kaute, me ngā kaihanga nui ngā hua HPLC motuhake hei whakaae i ngā kaiwhakarato hou.

He aha te hiranga o te ōritetanga o ia puranga?

I roto i te umanga tango tipu, ko te koretake tētahi o ngā tino take e ngaro ai ngā kirimana. Mena ka tutuki te tukunga tuatahi i ngā whakaritenga engari ka heke te parakore o te tuarua, ka rerekē rānei te āhua, me whakahaere anō pea e te kaihoko ngā whakamātautau pumau, me whakahou rānei. Mā te whakamātautau HPLC ka whakarite:

Ihirangi Hohe PūmauHe rite tonu te taumata parakore o ia rota.
Ngā Rekoata Ka Taea te WhaiMā ngā chromatogram me ngā COA puranga ka waihangahia he ara pepa mō ngā arotake.
Te Whakaiti i te Mōrearea o ngā AmuamuKa whakaiti i ngā tautohe mō ngā kereme parakore, ā, ka ārai i te whakakorenga i ngā wāhi tirotiro kounga.

Mō ngā waitohu tāpiringa, ko te tikanga o tēnei ōritetanga ka taea e rātou te pupuri i te tika o ngā tapanga hua me te kore he utu whakahou.

Me pēhea te whakamana a ngā kaihoko i te whakamātautau HPLC?

Ka whakaratohia e te kaiwhakarato pono:

COA me te Ōrau Parakore(hei tauira, “Luteolin 98.0% mā te HPLC”)
Kauwhata Chromatograme whakaatu ana i te tihi luteolin mārama i te wā pupuri tika
Tohutoro Tikanga Whakamātautau(hei tauira, momo pou, wāhanga neke, roangaru kimi)
Whakamātautau Tuatoru Motuhakemēnā e hiahiatia ana e te kaihoko

Mena kāore te kaiwhakarato e hiahia ki te tohatoha i ngā chromatogram, ki te homai rānei i ngā hua parakore me te kore e tohua te tikanga, he tohu hē tēnā.